Journal: Immunologic Research

Article Title: Hydroquinone predisposes for retinal pigment epithelial (RPE) cell degeneration in inflammatory conditions

doi: 10.1007/s12026-022-09300-0

Figure Lengend Snippet: The effect of hydroquinone (HQ 10 μM, 50 μM, 200 μM; 18 h) on the viability of IL-1α-primed ARPE-19 cells visualized using an Axio Vert A1 Zeiss microscope with AxioCam MRm camera and Zen 2011 program (Carl Zeiss Microscopy GmbH, Jena, Germany)

Article Snippet: After the treatments, cells were visually examined under an inverted light microscope (Axio Vert A1 Zeiss microscope with an AxioCam MRm camera and Zen 2011 program; Carl Zeiss Microscopy GmbH, Jena, Germany) before cell culture medium samples were collected into microtubes and centrifuged at 381 × g for 10 min, + 4 °C (Biofuge Fresco Heraeus Instruments, Newport Pagnell, UK).

Techniques: Microscopy

Journal: Immunologic Research

Article Title: Hydroquinone predisposes for retinal pigment epithelial (RPE) cell degeneration in inflammatory conditions

doi: 10.1007/s12026-022-09300-0

Figure Lengend Snippet: The effect of hydroquinone (HQ 10 μM, 200 μM; 18 h) on the levels of VEGF ( a ) and LDH ( b ) released by IL-1α-primed differentiated ARPE-19 cells. The condition of cells was also observed using an Axio Vert A1 Zeiss microscope with AxioCam MRm camera and Zen 2011 program ( c ). The data was collected from one experiment containing four parallel samples per group. Results were analyzed using the Mann–Whitney U -test and are shown as mean ± standard error of mean (SEM). * P < 0.05, ns, not significant. (Kruskal–Wallis test, ( a ) ** P < 0.01, ( b ) **** P < 0.0001)

Article Snippet: After the treatments, cells were visually examined under an inverted light microscope (Axio Vert A1 Zeiss microscope with an AxioCam MRm camera and Zen 2011 program; Carl Zeiss Microscopy GmbH, Jena, Germany) before cell culture medium samples were collected into microtubes and centrifuged at 381 × g for 10 min, + 4 °C (Biofuge Fresco Heraeus Instruments, Newport Pagnell, UK).

Techniques: Microscopy, MANN-WHITNEY